|[교육과학기술부·한국연구재단 지정 한림대학교 생명공학연구소]
◎ 제16차 생명공학연구소 정기세미나 개최 ◎
▶ 일 시 : 2012. 06. 20(수) 오전 11시
▶ 장 소 : 산학협력단 세미나실(12102호)
▶ 연 자 : 박용수 박사(한양대학교 의과대학)
▶ 주 최 : 생명공학연구소
▶ 강의제목 : Amelioration of diabetic micro-and macrovascular complications by TAT-mediated delivery of metallothionein and SOD
Diabetes mellitus and diabetic micro- and macrovascular complications (DCx) might be resulted from oxidative stress in various tissues including pancreatic islets and antioxidant treatment may improve underlying conditions. To circumvent their limited access into the biologic membrane, we made Tat-MT and Tat-SOD constructs applying cell penetrating peptide technologies, delivered to cardinal primary cultured cells of diabetes and diabetic complication development or their surrogate cell lines and studied cell viability in different injury conditions of high glucose, hypoxia and AGE treatment. The protective effect of Tat-MT and Tat-SOD in combination against the development of diabetes and complications development were also studied in OLETF rats. Tat-MT and Tat-SOD were successfully delivered to various cultured cells, and the intracellular activities of MT and SOD increased in line with the amount of protein delivered. These agents inhibited three different oxidative injuries as well as angiotensin II-mediated injuries and altered the expression of the downstream signaling molecules. In OLETF rats, a single intraperitoneal injection of Tat-MT and Tat-SOD resulted in delivery of these antioxidants to the various organs. Continuous treatment decreased the expression of ROS and downstream signaling molecules in various tissues and delayed the clinical development of diabetes and diabetic complications. The ameliorative effects of these agents against the development of micro- and macrovascular complications in vivo may be related to inhibition of ROS itself and downstream signaling pathways. We conclude that effective delivery of a combination antioxidant treatment may prevent and treat the pathophysiology in patients with diabetes and its complications.
▶ 연사 학력 및 경력
1980. 3 - 1986. 2 Seoul National University, College of Medicine M.D.
1988. 3 - 1990. 2 Seoul National University, College of Medicine Master of Medicine
1992. 3 - 1995. 2 Seoul National University, College of Medicine Ph. D
1995. 3 - present Professor Department of Internal Medicine, Hanyang University
1997. 8 - 1999, 7 Visiting Professor, Barbara Davis Center for Childhood Diabetes,University of Colorado Health Sciences Center
1991. 6. 21 Boehringer Mannheim Poster Award
1992. 9. 22 Eli Lilly Award
1995. 5. 16 Korean Soc. of Endocrinology, the best Researcher Award
1999 11.14 Kor. Diab. Ass., Seol-won Medical Award
2000. 5. 4 Korean Soc. of Endocrinology, the best Researcher Award
2002.12.13 Korean Ministry of Health and Welfare, The Best Researcher Award
2008.12. 3 Astra Zeneca/KHIDI VRI Research Grant Award 2008
연사분을 초청하여 제 16차 정기세미나를 개최하오니 많은 참석 부탁드리겠습니다.